Center of Regenerative Medicine

Prof. Dáša Čížková, DVM, DSc

Regenerative Medicine
Our Mission
The laboratory is oriented toward biomedicine, investigating the multiline potential, the genomic and proteomic profile of stem cells, and their metabolites obtained from adult and neonatal tissues. It uses modern in vitro and in vivo model systems and technologies of cell-tissue-3D-organoid engineering.
The main objective is to identify the most effective strategies for repairing damaged nervous tissue that is dysfunctional due to trauma, aging, and disease. We are achieving this through cell-based therapy, particularly with adult stem cells, genetically modified cells, or biomaterials seeded with stem cells capable of mechanical and trophic support. The main impact is on autologous sources of mesenchymal stem cells (MSC) isolated from bone marrow and adipose tissue. Both are safe, relatively easily accessible cells that are tolerated without a substantial risk of immune rejection. Our previous studies clearly confirmed their paracrine actions via released trophic and growth factors, cytokines, chemokines, metabolites, and bioactive lipids which stimulate endogenous regeneration and influence immune response. The application of conditioned media (CM) containing bioactive factors released by stem cells has the same regenerative potential as the direct transplantation of cells. Furthermore, CM contains extracellular vesicles (EVs) that mediate their paracrine effects by shuttling different types of RNAs (e.g., mRNA and miRNA) and proteins to recipient cells.
Delivery of CM-derived active factors may open the door to new clinical approaches for CNS treatment. Our research efforts focus on carefully selected cell-based or CM-based therapy for treating: i) CNS trauma, and ii) dementia associated with cognitive impairment in older dogs. The main strategy of cell-based therapy is to modulate inflammatory processes and improve recovery and axonal outgrowth, and synaptic structures in damaged nerve tissue.

International co-laboration

Research:
In collaboration with Laboratoire PRISM – INSERM U1192, University Lille1 in France we are elucidating the protein cargo of stem cells-derived EVs and identifying candidate proteins and biological signatures associated with the postulated therapeutic effects. Prof. Dasa Cizkova, DrSc. – an external member of PRISM laboratory
Cooperation in Ph.D. programs
Co-Tutorial Ph.D. Study Program with Laboratoire PRISM – INSERM U1192, University Lille1 in France/ 2016-2022. Prof. Michel Salzet, Ph.D./ Prof. Dasa Cizkova, DSc
Co-tutorial Ph.D. student – Finished (2016-2018) Mgr. Adriana Murgoci, Ph.D. Topic: Modulation of the microglia immune response in the context of the cross-talk between glioma and neuronal progenitor cells through extracellular vesicles. Thesis Defense: International board at UVLF Kosice/NiU SAV Bratislava Co-tutors – Prof. Michel Salzet, Ph.D./ Prof. Dasa Cizkova, DrSc. Members of the committee: Prof. MVDr. Jan Motlík, DrSc (Czech Republic), Ass.  Prof. Stefanii Mondell, Ph.D. (Italy), Prof. MVDr. Juraj Koppel, DrSc (Slovakia), Prof. Isabelle Fournier, Ph.D., Prof. Serge Nataf, Ph.D. (France), Ass. Prof. MVDr. Mangesh Bhide, Ph.D. (Slovakia).
Co-Tutorial Ph.D. student – In progress (2022-2024) Lydia Ziane Chaouche, Ph.D. Topic: Development and reprogramming of CAR macrophages for anti-tumor immunotherapy strategy. Co-tutors – Ass. Prof. Marie Duhamal, PhD/ Prof. Dasa Cizkova, DrSc.

Lab members

  • Tomáš Smolek, DVM, PhD
  • Veronika Cubínková, DVM, Ph.D.
  • Adriana-Natalia Murgoci, MSc., Ph.D.

Projects

  • APVV-SK-FR-2015-0018: Nová kombinovaná terapia na báze alginátových biomateriálov a trofických faktorov pre obnovu poranenej miechy (A novel combined therapy based on alginate biomaterials and trophic factors for spinal cord injury repair)
    • (1.1.2016 – 31.12.2017) Daša Čížková – Principal investigator
    • Financial support (APVV): 5290 €
  • APVV-15-0613: Štúdium funkčných bio-implantátov a bunková terapia pre regeneráciu CNS (Study of functional bio-implants and cell therapy for the CNS regeneration)
    • (1.7.2016-30.6.2020) Daša Čížková – Principal investigator
    • Financial support (APVV): 239 982 €
  • ERA-NET NEURON AxonRepair (NEURON9-FP-030): Spinal cord repair: releasing the neuron-intrinsic brake on axon regeneration (European Research Projects on External Insults to the Nervous System)
    • (01.1. 2017 – 31.12.2019) Daša Čížková – Principal investigator
    • Financial support (ERANET): 120 000 €
  • V4 Project 22020272, V4RM: Bridging the gap between science, education and enterprise in regenerative medicine. Applicant: Institute of Experimental Medicine CAS (Czech republic)
    • (01/10/2020–20/03/2022) Daša Čížková – Co-investigator

    Book chapters

    • ČÍŽKOVÁ, Dáša – MURGOCI, Adriana – Natalia – KREŠÁKOVÁ, Lenka – VDOVIAKOVÁ, Katarína – ČÍŽEK, Milan – SMOLEK, Tomáš – CUBINKOVÁ, Veronika – QUANICO, Jusal P. – FOURNIER, Isabelle – SALZET, Michel. Understanding Molecular Pathology along Injured Spinal Cord Axis: Moving Frontiers toward Effective Neuroprotection and Regeneration. In Essentials of Spinal Cord Injury. 2. dopl. vyd. – Rijeka : InTech, 2017. ISBN 978-953-51-5575-1, s. 1-21.

    Publications

    1. VIKARTOVSKÁ, Zuzana – FARBÁKOVÁ, Jana – SMOLEK, Tomáš – HANES, Jozef – ŽILKA, Norbert – HORŇÁKOVÁ, Ľubica – HUMENÍK, Filip – MALOVESKÁ, Marcela – HUDÁKOVÁ, Nikola – ČÍŽKOVÁ, Dáša. Novel diagnostic tools for identifying cognitive impairment in dogs: behavior, biomarkers, and pathology. In Frontiers in veterinary science. ISSN 2297-1769, 2021, vol. 7, no., art. no. 551895, p. [1-13]. (2020: 3.412 – IF, 3.588 – IF 5y, 31 – H-index, 0.877 – SJR, Q1 – SJR Best Q, Q1 – JCR Best Q). Dostupné na internete: <https://www.frontiersin.org/articles/10.3389/fvets.2020.551895/full>.
    2. VIKARTOVSKÁ, Zuzana – KURICOVÁ, Mária – FARBÁKOVÁ, Jana – LIPTÁK, Tomáš – MUDROŇOVÁ, Dagmar – HUMENÍK, Filip – MAĎARI, Aladár – MALOVESKÁ, Marcela – SYKOVÁ, Eva – ČÍŽKOVÁ, Dáša. Stem cell conditioned medium treatment for canine spinal cord injury: pilot feasibility study. In International Journal of Molecular Sciences. ISSN 1422-0067, 2020, vol. 21, no. 14, art. no. 5129, p. [1-21]. (2020: 5.924 – IF, 6.132 – IF 5y, 162 – H-index, 1.455 – SJR, Q1 – SJR Best Q, Q1 – JCR Best Q). (2020 – Current Contents).
    3. MURGOCI, Adriana – Natalia – CARDON, Tristan – ABOULOUARD, Soulaimane – DUHAMEL, Marie – FOURNIER, Isabelle – ČÍŽKOVÁ, Dáša – SALZET, Michel. Reference and ghost proteins identification in rat C6 glioma extracellular vesicles. In iScience. ISSN 2589-0042, 2020, vol. 23, no. 5, art. no. UNSP 101045, p. [1-10]. (2020: 5.458 – IF, 5.458 – IF 5y, 27 – H-index, 1.805 – SJR, Q1 – SJR Best Q, Q1 – JCR Best Q). (2020 – Current Contents).
    4. MURGOCI, Adriana – Natalia – DUHAMEL, Marie – RAFFO-ROMERO, Antonella – MALLAH, Khalil – ABOULOUARD, Soulaimane – LEFEBVRE, Christophe – KOBEISSY, Firas – FOURNIER, Isabelle – ŽILKOVÁ, Monika – MADEROVÁ, Denisa – ČÍŽEK, Milan – ČÍŽKOVÁ, Dáša – SALZET, Michel. Location of neonatal microglia drives small extracellular vesicles content and biological functions in vitro. In Journal of Extracellular Vesicles. ISSN 2001-3078, 2020, vol. 9, no. 1, art. no. 1727637, p. [1-18]. (2020: 25.841 – IF, 26.520 – IF 5y, 68 – H-index, 5.940 – SJR, Q1 – SJR Best Q, Q1 – JCR Best Q). (2020 – Current Contents).
    5. MURGOCI, Adriana – Natalia – BAČIAK, Ladislav – CUBINKOVÁ, Veronika – SMOLEK, Tomáš – TVRDÍK, Tomáš – JURÁNEK, Ivo – KAFKA, Jozef – ČÍŽKOVÁ, Dáša. Diffusion tensor imaging: Tool for tracking injured spinal cord fibres in rat. In Neurochemical Research. ISSN 0364-3190, 2020, vol. 45, no. 1, p. 180-187. (2020: 3.996 – IF, 3.858 – IF 5y, 116 – H-index, 1.102 – SJR, Q1 – SJR Best Q, Q2 – JCR Best Q). (2020 – Current Contents).
    6. ČÍŽKOVÁ, Dáša – MURGOCI, Adriana – Natalia – CUBINKOVÁ, Veronika – HUMENÍK, Filip – MOJŽIŠOVÁ, Zuzana – MALOVESKÁ, Marcela – ČÍŽEK, Milan – FOURNIER, Isabelle – SALZET, Michel. Spinal cord injury: Animal models, imaging tools and the treatment strategies. In Neurochemical Research. ISSN 0364-3190, 2020, vol. 45, no. 1, p. 134-143. (2020: 3.996 – IF, 3.858 – IF 5y, 116 – H-index, 1.102 – SJR, Q1 – SJR Best Q, Q2 – JCR Best Q). (2020 – Current Contents).
    7. HUMENÍK, Filip – ČÍŽKOVÁ, Dáša – ČIKOŠ, Štefan – LUPTÁKOVÁ, Lenka – MAĎARI, Aladár – MUDROŇOVÁ, Dagmar – KURICOVÁ, Mária – FARBÁKOVÁ, Jana – ŠPIRKOVÁ, Alexandra – PETROVOVÁ, Eva – CENTE, Martin – MOJŽIŠOVÁ, Zuzana – ABOULOUARD, Soulaimane – MURGOCI, Adriana – Natalia – FOURNIER, Isabelle – SALZET, Michel. Canine bone marrow-derived mesenchymal stem cells: genomics, proteomics and functional analyses of paracrine factors. In Molecular and Cellular Proteomics. ISSN 1535-9476, 2019, vol. 18, no. 9, p. 1824-1835. (2019: 4.870 – IF, 5.865 – IF 5y, 181 – H-index, 2.355 – SJR, Q1 – SJR Best Q, Q1 – JCR Best Q). (2019 – Current Contents).
    8. QUANICO, Jusal P. – HAUBERG-LOTTE, Lena – DEVAUX, Stephanie – LAOUBY, Zahra – MERIAUX, Celine – RAFFO-ROMERO, Antonella – ROSE, Mélanie – WESTERHEIDE, Leia – VEHMEYER, Jost – RODET, Franck – MAASS, Peter – ČÍŽKOVÁ, Dáša – ŽILKA, Norbert – CUBINKOVÁ, Veronika – FOURNIER, Isabelle – SALZET, Michel. 3D MALDI mass spectrometry imaging reveals specific localization of long-chain acylcarnitines within a 10-day time window of spinal cord injury. In Scientific Reports. ISSN 2045-2322, 2018, vol. 8, no. 1, article number 16083. (2018: 4.011 – IF, 4.525 – IF 5y, 179 – H-index, 1.414 – SJR, Q1 – SJR Best Q, Q1 – JCR Best Q). (2018 – Current Contents).
    9. DUHAMEL, Marie – ROSE, Mélanie – RODET, Franck – MURGOCI, Adriana – Natalia – ZOGRAFIDOU, Lea – RÉGNIER-VIGOUROUX, Anne – VANDEN ABEELE, Fabien – KOBEISSY, Firas – NATAF, Serge – PAYS, Laurent – WISZTORSKI, Maxence – ČÍŽKOVÁ, Dáša – FOURNIER, Isabelle – SALZET, Michel. Paclitaxel Treatment and Proprotein Convertase 1/3 (PC1/3) Knockdown in Macrophages is a Promising Antiglioma Strategy as Revealed by Proteomics and Cytotoxicity Studies. In Molecular and Cellular Proteomics. ISSN 1535-9476, 2018, vol. 17, č. 6, s. 1126-1143. (2018: 4.828 – IF, 5.914 – IF 5y, 181 – H-index, 2.807 – SJR, Q1 – SJR Best Q, Q1 – JCR Best Q). (2018 – Current Contents).
    10. MALOVESKÁ, Marcela – DANKO, Ján – PETROVOVÁ, Eva – KREŠÁKOVÁ, Lenka – VDOVIAKOVÁ, Katarína – MICHALICOVÁ, Alena – KOVÁČ, Andrej – CUBÍNKOVÁ, Veronika – ČÍŽKOVÁ, Dáša. Dynamics of Evans blue clearance from cerebrospinal fluid into meningeal lymphatic vessels and deep cervical lymph nodes. In Neurological Research. ISSN 0161-6412, 2018, vol. 40, č. 5, s. 372-380. (2018: 1.983 – IF, 1.773 – IF 5y, 75 – H-index, 0.632 – SJR, Q2 – SJR Best Q, Q3 – JCR Best Q). (2018 – Current Contents).
    11. ČÍŽKOVÁ, Dáša – CUBÍNKOVÁ, Veronika – SMOLEK, Tomáš – MURGOCI, Adriana – Natalia – DANKO, Ján – VDOVIAKOVÁ, Katarína – HUMENÍK, Filip – ČÍŽEK, Milan – QUANICO, Jusal P. – FOURNIER, Isabelle – SALZET, Michel. Localized intrathecal delivery of mesenchymal stromal cells conditioned medium improves functional recovery in a rat model of spinal cord injury. In International Journal of Molecular Sciences. ISSN 1422-0067, 2018, vol. 19, č. 3, p. 87-99. (2018: 4.183 – IF, 4.331 – IF 5y, 140 – H-index, 1.312 – SJR, Q1 – SJR Best Q, Q2 – JCR Best Q). (2018 – Current Contents)
    12. MURGOCI, Adriana – Natalia – ČÍŽKOVÁ, Dáša – MAJEROVÁ, Petra – PETROVOVÁ, Eva – MEDVECKÝ, Ľubomír – FOURNIER, Isabelle – SALZET, Michel. Brain-Cortex Microglia-Derived Exosomes: Nanoparticles for Glioma Therapy. In ChemPhysChem. ISSN 1439-4235, 2018, vol. 19, č. 10, s. 1205-1214. (2018: 3.077 – IF, 2.924 – IF 5y, 131 – H-index, 1.080 – SJR, Q1 – SJR Best Q, Q2 – JCR Best Q). (2018 – Current Contents).
    13. Devaux S, Cizkova D, Quanico J, Franck J, Nataf S, Pays L, Hauberg-Lotte L, Maass P, Kobarg JH, Kobeissy F, Meriaux C, Wisztorski M, Slovinska L, Blasko J, Cigankova V, Fournier I, Salzet M. Proteomic analysis of the spatio-temporal based molecular kinetics of acute spinal cord injury identifies a time- and segment-specific window for effective tissue repair. Mol Cell Proteomics, 2016 Aug;15(8):2641-70
    14. Corleto JA, Bravo-Hernández M, Kamizato K, Kakinohana O, Santucci C, Navarro MR, Platoshyn O,Cizkova D, Lukacova N, Taylor J, Marsala M. Thoracic 9 Spinal Transection-Induced Model of Muscle Spasticity in the Rat: A Systematic Electrophysiological and Histopathological Characterization. PLoS One. 2015 Dec 29;10(12):e0144642. doi: 10.1371/journal.pone.0144642. eCollection 2015.
    15. Grulova I, Slovinska L, Blaško J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D. Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair. Sci Rep. 2015 Sep 8;5:13702. doi: 10.1038/srep13702.
    16. Dasa Cizkova, Stephanie Devaux, Françoise Le Marrec-Croq, Julien Franck, Lucia Slovinska, Juraj Blasko, Jan  Rosocha, Timea Spakova, Christophe Lefebvre, Isabelle Fournier, Michel Salzet. Modulation properties of bone  marrow stromal cells released factors on activated microglia: in vitro  study, Scientific Reports ,4: 7514, 2014
    17. Kuricova M, Ledecky V, Liptak T, Madari A, Grulova I, Slovinska L, Nagyova M,Cizkova D. Oral administration of inosine promotes recovery after experimental spinal cord injury in rat. Neurol Sci. 35:1785–1791, 2014
    18. Cizkova D, Le Marrec-Croq F, Franck J, Slovinska L, Grulova I, Devaux S, Lefebvre C, Fournier I, Salzet M. Alterations of protein composition along the rostro-caudal axis after spinal cord injury: proteomic, in vitro and in vivo analyses.Front Cell Neurosci. 2014 Apr 17;8:105. doi: 10.3389/fncel.2014.00105. e Collection 2014.
    19. Nagyova M, Slovinska L, Blasko J, Grulova I, Kuricova M, Cigankova V, Harvanova D,Cizkova D. A comparative study of PKH67, DiI, and BrdU labeling techniques for tracing rat mesenchymal stem cells. In Vitro Cell Dev Biol Anim. 2014
    20. Grulova I, Slovinska L, Nagyova M, Cizek M, Cizkova D. The effect of hypothermia on sensory-motor function and tissue sparing after spinal cord injury.Spine J. 2013 Dec;13(12):1881-91.
    21. Grulova I, Slovinska L, Nagyova M, Cizek M, Cizkova D. The effect of hypothermia on sensory- motor function and tissue sparing after spinal cord injury. Spine J. 13(12) 1881-1891, 2013 Sep 5.
    22. Cizkova D, Slovinska L, Grulova I, Salzet M, Cikos S, Kryukov O, Cohen S. The influence of sustained dual-factor presentation on the expansion and differentiation of neural progenitors in affinity-binding alginate scaffolds. J Tissue Eng Regen Med J Tissue Eng Regen Med. 2015 Aug;9(8):918-29. doi: 10.1002/term.1797.
    23. PÉGO, Ana paula – KUBINOVÁ, Sarka – ČÍŽKOVÁ, Dáša – VANICKÝ, Ivo – MAR, Fernando Milhazes – SOUSA, Mónica Mendes – SYKOVA, Eva. Regenerative medicine for the treatment of spinal cord injury: more than just promises? In Journal of Cellular and Molecular Medicine, 2012, vol. 16, no. 11, p. 2564-2582. (4.125 – IF2011). (2012 – Current Contents). ISSN 1582-1838.
    24. Cizkova D, Novotna I, Slovinska L, Vanicky I, Jergova S, Rosocha J, Radonak J. Repetitive intrathecal catheter delivery of bone marrow mesenchymal stromal cells improves functional recovery in a rat model of contusive spinal cord injury. J Neurotrauma, 28(9):1951-61, 2011
    25. Novotna I, Slovinska L, Vanicky I, Cizek M, Radonak J, Cizkova D. IT Delivery of ChABC Modulates NG2 and Promotes GAP-43 Axonal Regrowth After Spinal Cord Injury. Cell Mol Neurobiol, (8):1129-39,  2011
    26. Mashkina AP, Cizkova D, Vanicky I, Boldyrev AA. NMDA receptors are expressed in lymphocytes activated both in vitro and in vivo. Cell Mol Neurobiol, 30(6):901-7, 2010
    27. Cizkova, D., Cizek, M., Nagyova, M., Slovinska, L., Novotna, I., Jergova, S., Radonak, J., Hlucilova, J., Vanicky, I. Enrichment of rat oligodendrocyte progenitor cells by magnetic cell sorting. Journal of Neuroscience  Methods, 184(1),  88-94, 2009.
    28. Cizkova, D., Nagyova, M., Slovinska, L., Novotna, I., Radonak, J., Cizek, M., Mechirova, E., Tomori, Z., Hlucilova, J., Motlik, J., Sulla, I. Jr., Vanicky, I. Response of ependymal progenitors to spinal cord injury or enhanced physical activity in adult rat. Cellular and Molecular Neurobiology, 29(6-7), 999-1013, 2009.
    29. Saganova, K., Orendacova, J., Sulla, I. Jr., Filipcik, P., Cizkova, D., Vanicky, I. Effects of long-term FK506 administration on functional and histopathological outcome after spinal cord injury in adult rat. Cellular and Molecular Neurobiology, 29(6-7), 1045-1051, 2009.
    30. Saganova, K., Orendacova, J., Cizkova, D., Vanicky, I. Limited minocycline neuroprotection after balloon-compression spinal cord injury in the rat. Neuroscience Letters, 433(3), 246-249, 2008.
    31. Jergova, S., Kolesar, D., Cizkova, D. Expression of c-Fos in the parabrachial nucleus following peripheral nerve injury in rats. European Journal of  Pain, 12(2), 172-179,2008.
    32. Cizkova, D., Kakinohana O., Kucharova K., Marsala S., Johe, , Hazel,  T., Hefferan, M.P., Marsala, M. Functional recovery in rats with ischemic paraplegia after spinal grafting of human spinal stem cells. Neuroscience, 147(2), 546-560, 2007.
    33. Jergova S., Cizkova D.: Microglial activation in different models of peripheral nerve injury of the rat. Journal of Molecular Histology, 38(3), 245-51, 2007.
    34. Cizkova, D., Rosocha, J., Vanicky, I., Jergova, S., Cizek, M. Transplants of human mesenchymal stem cells improve functional recovery after spinal cord injury in the rat. Cellular and Molecular  Neurobiology, 26(7-8), 1167-1180, 2006.
    35. Cizkova, D., Rosocha, J., Vanicky, I., Radonak, J., Galik, J., Cizek, M. Induction of mesenchymal stem cells leads to HSP72 synthesis and higher resistance to oxidative stress. Neurochemical Research, 31(8), 1011-1120, 2006.
    36. Saganova, K., Burda, J., Orendacova, J., Cizkova, D., Vanicky, I. Fluoro-Jade B staining following zymosan microinjection into the spinal cord white matter. Cellular and Molecular  Neurobiology, 26(7-8), 1463-1473, 2006.
    37. Allen, J.W., Mantyh, P.W., Horais, K., Tozier, N., Rogers, S.D., Ghilardi, J.R., Cizkova, D., Grafe, M.R., Richter, P., Lappi, D.A., Yaksh, T.L. Safety evaluation of intrathecal substance P-saporin, a targeted neurotoxin, in dogs. Toxicological Sciences, 91(1), 286-298, 2006.
    38. Racekova, E., Martoncikova, M., Mitruskova, B., Cizkova, D., Orendacova, J. Age-related changes of NADPH-diaphorase positivity in the rat rostral migratory stream. Cellular and Molecular  Neurobiology, 25(7), 1093-1105, 2005.
    39. Cizkova, D., Lukacova, N., Marsala, M., Kafka, J., Lukac, I., Jergova, S., Cizek, M., Marsala, J. Experimental cauda equina compression induces HSP70 synthesis in dog. Physiological Research, 54(3), 349-356, 2005.
    40. Orendacova, J., Ondrejcak, T., Kucharova, K., Cizkova, D., Jergova, S., Mitruskova, B., Racekova, E., Vanicky, I., Marsala, J. Fluoro-Jade B evidence of induced ischemic tolerance in the rat spinal cord ischemia: physiological, neurological and histopathological consequences. General Physiology and Biophysics, 24(1), 75-87, 2005.
    41. Jergova, S., Cizkova, D. Long-term changes of c-Fos expression in the rat spinal cord following chronic constriction injury. European Journal of  Pain, 9(3), 345-354, 2005.
    42. Marsala, M., Kakinohana, O., Hefferan, M.P., Cizkova, D., Kinjoh, K., Marsala, S. Synaptogenesis and amino acid release from long term embryonic rat spinal cord neuronal culture using tissue culture inserts. Journal of Neuroscience Methods, 141(1), 21-27, 2005.
    43. Marsala, M., Kakinohana, O., Yaksh, T.L., Tomori, Z., Marsala, S., Cizkova, D. Spinal implantation of hNT neurons and neuronal precursors: graft survival and functional effects in rats with ischemic spastic paraplegia. European Journal of Neuroscience, 20(9), 2401-2014, 2004.
    44. Kakinohana, O., Cizkova, D., Tomori, Z., Hedlund, E., Marsala, S., Isacson, O., Marsala, M. Region-specific cell grafting into cervical and lumbar spinal cord in rat: a qualitative and quantitative stereological study. Experimental Neurology, 190(1), 122-132, 2004.
    45. Marsala, J., Lukacova, N., Cizkova, D., Lukac, I., Kucharova, K., Marsala, M. Premotor nitric oxide synthase immunoreactive pathway connecting lumbar segments with the ventral motor nucleus of the cervical enlargement in the dog. Journal of Chemical Neuroanatomy, 27(1), s. 43-54, 2004.
    46. Lukacova, N., Kafka, J., Cizkova, D., Marsala, M., Marsala, J. The effect of cauda equina constriction on nitric oxide synthase activity. Neurochemical Research, 29(2), 429-439, 2004.
    47. Cizkova, D., Carmel, J.B., Yamamoto, K., Kakinohana, O., Sun, D., Hart, R.P., Marsala, M. Characterization of spinal HSP72 induction and development of ischemic tolerance after spinal ischemia in rats. Experimental Neurology, 185(1),  97-108, 2004.
    48. Tokumine, J., Kakinohana, O., Cizkova, D., Smith, D.W., Marsala, M. Changes in spinal GDNF, BDNF, and NT-3 expression after transient spinal cord ischemia in the rat Journal of  Neuroscience  Research, 74(4), 552-561, 2003.
    49. Sasara, T., Cizkova, D., Mestril, R., Galik, J., Sugahara, K., Marsala, M. Spinal heat shock protein (70) expression: effect of spinal ischemia, hyperthermia (42 degrees C)/hypothermia (27 degrees C), NMDA receptor activation and potassium evoked depolarization on the induction. Neurochemistry International, 44(1), 53-64, 2004.
    50. Marsala, J., Kafka, J., Lukacova, N., Cizkova, D., Marsala M., Katsube N. Cauda equina syndrome and nitric oxide synthase immunoreactivity in the spinal cord of the dog. Physiological Research, 52(4), 2003, s. 481-496.
    51. Burda, J., Hrehorovska, M., Bonilla, L.G., Danielisova, V., Cizkova, D., Burda, R., Nemethova, M., Fando, J.L., Salinas, M. Role of protein synthesis in the ischemic tolerance acquisition induced by transient forebrain ischemia in the rat. Neurochemical Research, 28(8), 2003, s. 1213-1219.
    52. Hayes, C.S., Mulkmus, S.A., Cizkova, D., Yaksh, T.L., Hua, X.Y. A double-lumen intrathecal catheter for studies of modulation of spinal opiate tolerance. Journal of Neuroscience  Methods, 30;126(2), 2003 s.165-173.
    53. Marsala, J., Marsala, M., Lukacova, N., Ishikawa, T., Cizkova, D. Localization and distribution patterns of nicotinamide adenine dinucleotide phosphate diaphorase exhibiting axons in the white matter of the spinal cord of the rabbit. Cellular and Molecular Neurobiology, 23(1), 2003, s. 57-92.
    54. Horais, K., Hruby, V., Rossi, S., Cizkova, D., Meschter, C., Dorr, R., Yaksh, T.L. Effects of chronic intrathecal infusion of a partial differential opioid agonist in dogs. Toxicological Sciences, 71(2), 2003, s. 263-275.
    55. Jergova, S., Cizkova, D., Orendacova, J, Cizek, M., Marsala, J. Localization of c-Fos protein in the rat spinal cord after carrageenan treatment. Acta Histochemica, 104(4), 2002, s. 381-385
    56. Lukacova, N., Cizkova, D., Krizanova, O., Pavel, J., Marsala, M., Marsala, J. Peripheral axotomy affects nicotinamide adenine dinucleotide phosphate diaphorase and nitric oxide synthases in the spinal cord of the rabbit. Journal of Neuroscience  , 71(2), 2003, s. 300-313.
    57. Cizkova, D., Marsala, J., Lukacova, N., Marsala, M., Jergova, S., Orendacova, J., Yaksh, T.L. Localization of N-type Ca2+ channels in the rat spinal cord following chronic constrictive nerve injury. Experimental Brain Research, 147(4), 2002, s. 456-463.
    58. Marsala, J., Lukacova, N., Cizkova, D., Kafka, J., Katsube, N., Kucharova, K., Marsala, M. The case for the bulbospinal respiratory nitric oxide synthase-immunoreactive pathway in the dog. Experimental Neurology, 177(1), 2002, s. 115-132.
    59. Lukacova, N., Cizkova, D., Marsala, M., Lukac, I., Marsala, J. The regional distribution of nitric oxide synthase activity in the spinal cord of the dog. Brain Research Bulletin, 58(2), 2002, s. 173-178.
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